VIBRATOMOS

VIBRATOME CLASSIC STARTER AND PROFESSIONAL CE

The original Vibratome Semi-Automatic Tissue Sectioning System has maintained the reputation of a rock solid tissue sectioning system. Researchers from around the globe have relied on the Vibratome 1000 Classic for their fresh and fresh fixed tissue sectioning needs for over 20 years.

With the Vibratome Automatic Tissue Sectioning Systems, the biological viability of the tissue is maintained, with no embedding or freezing to change the characteristics of specimens before further examination. They cut fresh or fixed human, animal, plant tissue and even some non-biological materials, evenly without distortion. The unit is available in four different configurations.

Detailed Description

Typical applications include: enzyme histochemistry, tissue pathology, autoradiology, water soluble compounds, immunocytochemistry, immunohistochemistry, fluorescent microscopy, tissue pathology, botanical studies, and preparation step prior to electron microscopy.

The original Vibratome® semi-automatic fresh or fixed tissue sectioning system has maintained a long reputation as a rock solid tissue sectioning system. Researchers from around the globe have relied on the Vibratome® 1000 Classic for their fresh tissue sectioning needs for over 30 years.

The Vibratome® 1000 Plus is built on the same rock solid mechanical system as researchers have grown to depend on for years. The Vibratome® 1000 Plus utilizes the same mechanical sectioning components with enhanced specifications and features such as: smaller section thickness increments, increased sectioning speed, faster return stroke speed, increased maximum sectioning range, drain function, standard dual bath, and finer height adjustment. A stroke pause switch and adjustable light and magnifier are also part of the Vibratome® 1000 Plus system.

All Vibratomes® are supplied complete with: Blade Angle Indicator, Vise-Type Specimen Holder, Specimen Mounting Blocks, Dust Cover, Magnifying Lamp, Fluorescent Tube, Specimen Adhesive, Razor Blades, Spare Fuse and Operating Instructions. Other optional accessories include: Rotating Stage Assembly, Specimen Mounts, V-Block Adapter, Section Mounting Device, Specimen Tray, Sapphire Knife, Glass Knife Adapter and Specimen Retrievers.

Discussion in Depth

The Vibratome® series of fresh tissue sectioning systems allows sectioning of fresh or fixed tissue without freezing or embedding. The patented vibrating blade principle moves the sectioning blade edge in a reciprocating accurate path as it penetrates the specimen. The reduced effective edge angle from the transverse movements, with the lateral distribution of the cutting edge penetrating pressure, minimize elastic deformation of soft tissue specimens that are simply held or capsulated in position during the sectioning operation.

Uniform sections can be made of delicate soft, tissue specimens. Sections are free of observable compressive distortion in the direction of cut, as would be typical with conventional microtomes using oscillating blade actions. Preservation of ultrastructure is maintained in the sections from a lack of cellular damage on their surfaces. Sectioning takes place in a liquid bath. The liquid serves to lubricate the sectioning blade, to maintain specimen temperature, enhance or preserve desirable characteristics of the specimen, and facilitate the easy retrieval of the sections generated.

Using the technique described, the Vibratome® series of microtomes deliver superior performance using ordinary injector type razor blades. Sectioning of uniform fresh tissue sections can be cut from 20 µm up, although thinner sections may be realized, depending on the type of tissue involved, and operator technique. For conventional use, expensive blades made from exotic materials are not required, cutting the costs of normal operation and maintenance. If a more precise and finer tissue slice is required, use a sapphire blade which provides slices as thin as 6 µm. All Vibratomes® can work with standard, glass, sapphire or injector knife holders.

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