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MATERIAL
DE MICROSCOPIA ELECTRÓNICA: Kit de Inclusión 
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Araldite 502
Embedding Kit: The
Araldite 502 kit with BDMA accelerator is equally efficient as the
same kit using DMP-30. BDMA has a lower viscosity and diffuses more
rapidly into tissues, porous specimens and rough surfaces. The
Araldite 502 resins have better beam stability than the Eponate
resins. BDMA is used in 3% concentrations versus 1.5 to 2% for DMP-30.
Araldite kit may be used alone or mixed with other epoxies. Curing
times are 16 to 24 hours at a temperature of 60°C or 2 to 3 hours
using the PELCO BioWave®.
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Eponate 12™
Kit, with BDMA: Aliphatic
epoxy resin based on di- and tri-glydicyl ethers of glycerol with a
significantly lower viscosity compared to Araldite 502. BDMA
(<3%, see notes preceding) is used as the accelerator since it
has lower viscosity and diffuses into tissues more rapidly. (See
references below).
The viscosity of Eponate™ 12 at 25ºC is 15-21cp, WPE is 140–145
and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC
include DDSA (290cp) and NMA (225-275cp). Eponate™ 12 is
hygroscopic.
Curing time is 16 to 24 hours at a temperature of 60°C or 2 to 3
hours using the PELCO BioWave®.
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JB-4®
Plus Embedding Kit: JB-4
Plus® Embedding Kit for light microscopy is a water soluble, GMA
based, plastic resin kit intended for use in the preparation of
embedded samples for high resolution light microscopy. Its cooler
acting accelerator system offers greater heat protection of tissue
components. It yields semi-thin sections (0.5 - 2µm) with excellent
morphological preservation. Crystal clear non-yellowing casts are
obtained at room temperature in 90 minutes or less.
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Lowicryl®
Embedding Kit Polar, K11M:
New, pre-mixed, ready to use MonoStep® Lowicryl® Embedding Media
saves time and minimizes chemical contact, besides being much lower
in price. These products are extensively used in
Immunohistochemistry and Immunocytochemistry. The new MonoStep®
products are based on the popular Lowicryl® K4M and HM20 products
for low temperature embedding or freeze substitution. UV curing may
also be employed; between 0ºC and 30ºC a photoinitiator is
suggested (benzoin ethyl ether, 0.1g).
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PELCO®
Acrylic Embedding Resin Kit
A unique acrylic resin
modeled after the UNICRYL® acrylic resin. The advantage of this
resin is that the catalyst is supplied separate from the resin
components. Shelf life is improved and heat induced changes during
shipping are eliminated.
This resin is ideally suited for LM and EM applications (immunocytochemistry,
ultrastructural preservation, in situ hybridization with good
cutting properties for either thick or thin sectioning). Unique
cutting properties have been attributed to this resin formulation.
It is felt that proteins, nucleic acids and macromolecules are
revealed at the surface during sectioning. It is a hydrophilic resin
which exhibits unique staining properties at the light level for a
wide range of stains (i.e. Hemotoxylin, Silver Methenamine, Light
Green, Eosin, Safranin, Toluidine Blue).
The resin contains Styrene Monomer (hazard, see MSDS), Monomeric (Meth)-acrylate
Esters with Benzoyl Peroxide supplied as the initiator. Mixing of
the initiator is required prior to use. The resin is best stored at
-20°C after the addition of the initiator but the resin will remain
liquid down to -50°C. It is a pale yellow liquid with a pungent
odor that can be cured with either heat or UV radiation. Best
results are attained when the resin is microwave cured. The resin
should be capped during polymerization due to evaporation. Use
proper gloves, fume hood and eye protection.
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